Fas Ligand (FASL) (FITC)

Applications: \nSuitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

F0019-66T

Size

100Tests

Applications

FC, ICC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.02% sodium azide. Conjugated to FITC.

P Type

Mab

Purity

(same/more than)95% (by SDS-PAGE).

Isotype

IgG2a

References

1. Characterization of the non-functional Fas ligand of gld mice: M. Hahne, et al.; Int. Immunol. 7: 1381 (1995). 2. CD4+ T cells reactivated with superantigen are both more sensitive to FasL-mediated killing and express a higher level of FasL: J.K. Wang, et al.; Cell. Immunol. 179: 153 (1997). 3. Transgenic expression of CD95 ligand on thyroid follicular cells confers immune privilege upon thyroid allografts: L. Tourneur, et al.; J. Immunol. 167: 1338 (2001). 4. Mature T cells of autoimmune lpr/lpr mice have a defect in antigen-stimulated suicide: H. Russell, et al.; PNAS 90: 4409 (1993). 5. Cytolytic T-cell cytotoxicity is mediated through perforin and Fas lytic pathways: B. Lowin, et al.; Nature 370: 650 (1994). 6. A role for CD95 ligand in preventing graft rejection: D. Bellgrau, et al.; Nature 377: 630 (1995). 7. Fas ligand-induced apoptosis as a mechanism of immune privilege: T.S. Griffith, et al.; Science 270: 1189 (1995). 8. Apoptosis by death factor: S. Nagata; Cell 88: 355 (1997)

Additional Info

Recognizes mouse FasL.