Fatty Acid Binding Protein 5, Epidermal, Recombinant, Human (EFABP, FABP5, mal1, MGC133132)

Adipocyte fatty acid binding protein FABP5 is a 15kD member of the intracellular fatty acid binding protein (FABP) family, which is known for the ability to bind fatty acids and related compounds ( bile acids or retinoids). in an internal cavity. The fatty acid binding proteins aP2 (fatty acid binding protein [FABP]-4) and mal1 (FABP5) are closely related and both are expressed in adipocytes. Absence of FABP5/mal1 resulted in increased systemic insulin sensitivity in two models of obesity and insulin resistance. Adipocytes isolated from mal1-deficient mice also exhibited enhanced insulin-stimulated glucose transport capacity. In contrast, mice expressing high levels of mal1 in adipose tissue display reduced systemic insulin sensitivity.\n\nSequence:\nMATVQQLEGRWRLVDSKGFDEYMKELGVGIALRKMGAMAKPDCIITCDGKNLTIKTESTLKTTQFSCTLGEKFEETTADGRKTQTVCNFTDGALVQHQEWDGKESTITRKLKDGKLVVECVMNNVTCRIYEKVE\n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications have not been tested.\nOptimal dilutions to be determined by researcher\n\nStorage and Stability:\nStore lyophilized protein at -20 degrees C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degrees C for a limited period of time; it does not show any change after two weeks at 4 degrees C. The lyophilized protein remains stable at least 12 months when stored at -20 degrees C.

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SPECIFICATIONS

Catalog Number

F0019-73F

Size

100ug

Applications

ELISA, WB

Form

Supplied as a lyophilized powder from PBS. Reconstitute with 0.2ml of sterile ddH20 and let the lyophilized pellet dissolve completely.

Purity

>90% (SDS-PAGE). Two-step procedure using size exclusion chromatography before and after refolding.

References

1. Maeda K, Uysal KT, Makowski L, Gorgun CZ, Atsumi G, Parker RA, Bruning J, Hertzel AV, Bernlohr DA, Hotamisligil GS. Role of the fatty acid binding protein mal1 in obesity and insulin resistance. Diabetes. 2003 Feb;52(2):300-7.\n2. Fisher RM, Hoffstedt J, Hotamisligil GS, Thorne A, Ryden M. Effects of obesity and weight loss on the expression of proteins involved in fatty acid metabolism in human adipose tissue. Int J Obes Relat Metab Disord. 2002 Oct;26(10):1379-85.\n3. Jenkins-Kruchten AE, Bennaars-Eiden A, Ross JR, Shen WJ, Kraemer FB, Bernlohr DA. Fatty acidbinding protein-hormone-sensitive lipase interaction. Fatty acid dependence on binding.J Biol Chem. 2003 Nov 28;278(48):47636-43

Additional Info

The amino acid sequence of the recombinant human EFABP is 100% homologous to the amino acid sequence of the human EFABP.