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Fatty Acid Binding Protein 5, Epidermal, Recombinant, Human (EFABP, FABP5, mal1, MGC133132)

Cat no: F0019-73J

Fatty Acid Binding Protein 5, Epidermal, Recombinant, Human (EFABP, FABP5, mal1, MGC133132)

FABP5 is a member of the intracellular fatty acid binding protein (FABP) family, which is known for the ability to specifically bind fatty acids (FAs) with high affinity for stearic and linoleic acids. FABP5 is expressed in endothelial cells of the microvasculature of the placenta, heart, skeletal muscle, small intestine, lung, and renal medulla. FABP4 and FABP5 are closely related and both are expressed in adipocytes. Absence of FABP5 resulted in increased systemic insulin sensitivity in two models of obesity and insulin resistance.\n\nRecombinant human FABP5, aa1-135, was expressed in E. coli and purified by conventional chromatography.\n\nAA Sequence:\nMATVQQLEGR WRLVDSKGFD EYMKELGVGI ALRKMGAMAK PDCIITCDGK NLTIKTESTL KTTQFSCTLG EKFEETTADG RKTQTVCNFT DGALVQHQEW \nDGKESTITRK LKDGKLVVEC VMNNVTCTRI YEKVE\n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications have not been tested.\nOptimal dilutions to be deter\n\nRecommended Dilutions:\nOptimal dilutions to be determined by researchermined by researcher\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F0019-73J

Size

100ug

Applications

ELISA, WB

Form

Supplied as a liquid in 20mM Tris pH 8.0, 1mM DTT, 20% glycerol.

Purity

~90% (SDS-PAGE)

References

1. Hagens G., et al: (1999) Biochem.J ; 339 ( Pt 2): 419-27 2. Hohoff C., et al: (1999) Biochemistry ; 38 (38): 12229-39

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