FCRLB, Recombinant, Human (FCRL2, FCRY, FREB2, Fc Receptor-like B, Fc Receptor homolog expressed in B cells Protein 2, Fc Receptor-like and Mucin-like Protein 2, Fc Receptor-like Protein 2, Fc Receptor-related Protein Y, FCRLM2, FCRLY, FcRY, FLJ31052, FRE

FCRLB, also known as FCRL2, FCRY, and FREB2, is a 60kD protein with sequence homology to classical Fc receptors. There are at least six type 1 transmembrane FCRL proteins and two that lack transmembrane segments. Each family member contains between three and nine immunoglobulin-like domains. FCRL proteins are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation. According to our testing, FCRLB binds to purified human IgG with high affinity. Human FCRLB shares 82% and 85% aa sequence identity with mouse and rat FCRLB, respectively. It contains a putative signal peptide, three immunoglobulin-like domains, and a mucin-like stalk that is rich in Pro, Ser, and Thr residues. The stalk region also contains di-Leu motifs and an unpaired cysteine. Alternative splicing generates isoforms with deletions in the putative signal peptide and substitutions and/or truncations in the third Ig -like domain. When expressed in transfectants, FCRLB is not secreted but shows a diffuse intracellular localization. FCRLB is expressed at a low level in placenta and in B lineage cells of the germinal center. It is upregulated in B cells by BAFF and LPS. Among non-hematopoietic cells, FCRLB is expressed in fibroblasts, melanocytes, and melanoma. It is preferentially expressed in nonproliferating cells and at the onset of apoptosis.\n\nSource:\nMurine myeloma cell line, NS0-derived. Ala18-Ser426, with a C-terminal 6x-His tag.\n\nMolecular Weight: 65-70kD (SDA-PAGE, reducing conditions) \n\nBiological Activity:\nMeasured by the ability to bind human IgG with an estimated KD <120nM. \n\nEndotoxin: (same/less than)1EU/ug (LAL) \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute with 100ug/ml PBS. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F0025-73

Size

50ug

Form

Supplied as a lyophilized powder from PBS, EDTA. Carrier free. Reconstitute with 100ug/ml PBS.

Purity

(same/more than)90% (SDS-PAGE)

References

1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525. 2. Maltais, L.J. et al . (2006) Nat. Immunol. 7:431. 3. Chikaev, N.A. et al . (2005) Genomics 85:264. 4. Wilson, T.J. and M. Colonna (2005) Genes Immun. 6:341. 5. Masuda, K. et al . (2005) Gene 363:32.