Ferritin, Human, BioAssay(TM) ELISA Kit

One of the most prevalent disorders of man is the dietary deficiency of iron and the resulting anemia. Therefore, the assays of iron, total iron binding capacity and other assessments of iron compounds in the body are clinically significant. \n \nIron-storage compounds in the body include hemoglobin, hemosiderin, myoglobulin and the cytochromes. In most tissues, ferritin is a major iron-storage protein. Human ferritin has a molecular weight of approximately 450kD, and consists of a protein shell around an iron core; each molecule of ferritin may contain as many as 4,000 iron atoms. Under normal conditions, this may represent 25% of the total iron found in the body. In addition, ferritin can be found in several isomers. \n \nHigh concentrations of ferritin are found in the cytoplasm of the reticuloendothelial system, the liver, spleen and bone marrow. Methods previously used to measure iron in such tissues are invasive, cause sample trauma and lack adequate sensitivity. The measurement of ferritin in serum is useful in determining changes in body iron storage, and is non-invasive with relatively little sample discomfort. Serum ferritin levels can be measured routinely and are particularly useful in the early detection of iron-deficiency anemia in apparently healthy people. \n \nSerum ferritin measurements are also clinically significant in the monitoring of the iron status of pregnant women, blood donors, and renal dialysis samples. High ferritin levels may indicate iron overload without apparent liver damage, as may be noted in the early stages of idiopathic hemochromatosis. Ferritin levels in serum have also been used to evaluate clinical conditions not related to iron storage, including inflammation, chronic liver disease, and malignancy. \n\nThe Ferritin, Human, BioAssay(TM) ELISA Kit provides a rapid, sensitive and reliable assay. The antibodies developed for the test will determine a minimal concentration of sample ferritin of 5ng/ml. There is minimal cross-reativity with sample serum albumin, alpha-fetoprotein, sample hemoglobin, sample transferrin, and ferric chloride. \n\nKit Components:\nF4015-18A: Microtiter Plate: 1x96 wells \nF4015-18B: Standard 0ng/ml: 1x1ml\nF4015-18C: Standard 10ng/ml: 1x1ml\nF4015-18D: Standard 50ng/ml: 1x1ml\nF4015-18E: Standard 100ng/ml: 1x1ml\nF4015-18F: Standard 400ng/ml: 1x1ml\nF4015-18G: Standard 800ng/ml: 1x1ml\nF4015-18H: Enzyme Conjugate: 1x12ml \nF4015-18J: TMB Substrate: 1x12ml \nF4015-18K: Stop Solution, 2N HCl: 1x12ml \nF4015-18L: Wash Solution, 50X: 1x15ml\n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F4015-18

Size

1Kit

Applications

ELISA

Reactivities

Hum

References

1. White, D.; et al., A.m. J. Clin. Path. 72: 346; 1986 2. Valberg, L. CMAJ. 122:1240; 1980 3. Forman, D. and Parker, S. Ann. Clin. Lab. Sci. 10: 345; 1980. 4. Hazard, J.T.; et al., J. Blood. 49:139; 1977 5. Smimes, M.A.; Addiego, Jr .J.E. and Dallman, P.R. Blood. 43: 581; 1974.