Fibroblast Growth Factor 2, Basic, Neutralizing (FGF2b)

Basic fibroblast growth factor (bFGF or FGF2) is a member of the fibroblast growth factor family. In normal tissue, bFGF is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. It stays membrane-bound as long as there is no signal peptide. It has been hypothesized that, during both wound healing of normal tissues and tumor development, the action of heparan sulfate-degrading enzymes activates bFGF, thus mediating the formation of new blood vessels. Additionally, bFGF is a critical component of human embryonic stem cell culture medium.\n\nApplications: \nSuitable for use in Neutralization. Not suitable for Western Blot, use F4210-21 Fibroblast Growth Factor 2, Basic (FGF2b). Other applications have not been tested.\n\nRecommended Dilutions:\nNeutralization of bFGF Activity Assay: 2.5ug/ml neutralizes >40% growth of bovine FBHE cells simulated with 5ng/ml of FGF-2/bFGF. \nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nP3X63-Ag8.U1 myeloma cells with spleen cells from Balb/c mice. \n\nRecommended Positive Control: \nFetal liver or kidney tissue\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

F4210-22

Size

500ug

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid in TBS. No preservatives added.

P Type

Mab

Purity

Purified by ammonium sulfate precipitation and DEAE-cellulose chromatography.

Isotype

IgG1,k

References

1. Matsuzaki, K., Proc. Natl. Acad. Sci. USA, 86: 9911 (1989). 2. Korff, T. et al., FASEB J. 15:447-457 (2001). 3.Palamakumbura ,A. H. et al., J Biol Chem 278:30781-30787 (2003)

Additional Info

Recognizes the non-denatured form of bovine basic FGF2. Does not crossreact with bovine acidic FGF (HBGF-1). Species Crossreactivity: human, rat and mouse.