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Fibroblasts

Cat no: F4213

Fibroblasts

Applications:\nSuitable for use in Immunofluorescence, FACS, and the assessment of thymic epithelial culture contamination with fibroblasts and the identification of cells of mesodermal origin in the fetus. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunofluorescence (Acetone fixed tissue): 1:100-1:200 \nFACS: 1:100 using fresh or acetone fixed tissue; do not use trypsin to prepare adherent cell lines, use a non-enzymatic detactment procedure.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F4213

Size

100ug

Applications

FC, ICC, IF, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 1mg/ml BSA, 10mM sodium azide

P Type

Mab

Purity

Purified by ion exchange chromatography.

Isotype

IgG1

References

1.Haynes B.F. Adv. Immunol. 81

Additional Info

Recognizes human fibroblasts in tissue as well as cultured fibroblasts. On skin sections the antibody reacts with stratum basal epidermal cells. The antigen appears to be a marker for mesodermally dervied (mesenchymal) human connective tissue and tumors derived from such tissue (e.g. fibrosarcomas) {Haynes et al, 1984}. Useful for the assessment of thymic epithelial culture contamination with fibroblasts, and the identification of cells of mesodermal origin in the fetus.

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