Fis1 (TTC11, Mitochondrial Fission 1 Protein, FIS1 Homolog, hFis1, Tetratricopeptide Repeat Protein 11, TPR Repeat Protein 11, CGI-135)

Fis1, a 17kD integral membrane protein, is a novel member of mitochondrial fission machinery. It consists of a central leucine-zipper domain, a coiled-coil region, a tetratricopeptide repeat domain and a C-terminal transmembrane domain. It is a human orthologue of Fis1p (S.cerevisiae) and is localized in the outer mitochondrial membrane. Fis1 acts as a pro-apoptotic factor and plays a vital role in the regulation of mitochondrial fission. It interacts with DLP1 and Mdv1p and facilitates Dnm1p recruitment to the functional fission complex, thereby inducing apoptosis. Role of Fis1 has also been implicated in growth and division of peroxisomes. Loss of Fis1 inhibits apoptosis and results in elongation of mammalian mitochondria.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nImmunohistochemistry: Methacarn fixed, paraffin-embedded\nOptimal dilutions to be determined by the researcher. \n\nPositive Control:\nHeLa (human), NIH 3T3 (mouse)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F4514-70B

Size

200ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Serum.

Isotype

IgG

References

1. James,D.I., Parone,P.A., Mattenberger,Y. and Martinou,J.C. hFis1, a novel component of the mammalian mitochondrial fission machinery J. Biol. Chem. 278 (38), 36373-36379 (2003). 2. Dohm,J.A., Lee,S.J., Hardwick,J.M., Hill,R.B. and Gittis,A.G. Cytosolic domain of the human mitochondrial fission protein fis1 adopts a TPR fold. Proteins 54 (1), 153-156 (2004). 3. Yu et.al Journal of Cell Science 118, 4141-4151 (2005) 4. Karren et.al JCB, 171 (2), 291-301 (2005) 5. Koch et.al MCB, 16 (11); 5077-5086 (November 2005).

Additional Info

Recognizes human Fis1. Species sequence homology: mouse, rat, chimpanzee, canine.