Home  >  Products  >  Fis1 (TTC11, Mitochondrial Fission 1 Protein, FIS1 Homolog, hFis1, Tetratricopeptide Repeat Protein 11, TPR Repeat Protein 11, CGI-135)
Fis1 (TTC11, Mitochondrial Fission 1 Protein, FIS1 Homolog, hFis1, Tetratricopeptide Repeat Protein 11, TPR Repeat Protein 11, CGI-135)

Fis1 (TTC11, Mitochondrial Fission 1 Protein, FIS1 Homolog, hFis1, Tetratricopeptide Repeat Protein 11, TPR Repeat Protein 11, CGI-135)

Cat no: F4514-70B

Supplier: United States Biological
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Fis1, a 17kD integral membrane protein, is a novel member of mitochondrial fission machinery. It consists of a central leucine-zipper domain, a coiled-coil region, a tetratricopeptide repeat domain and a C-terminal transmembrane domain. It is a human orthologue of Fis1p (S.cerevisiae) and is localized in the outer mitochondrial membrane. Fis1 acts as a pro-apoptotic factor and plays a vital role in the regulation of mitochondrial fission. It interacts with DLP1 and Mdv1p and facilitates Dnm1p recruitment to the functional fission complex, thereby inducing apoptosis. Role of Fis1 has also been implicated in growth and division of peroxisomes. Loss of Fis1 inhibits apoptosis and results in elongation of mammalian mitochondria. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Immunohistochemistry: Methacarn fixed, paraffin-embedded Optimal dilutions to be determined by the researcher. Positive Control: HeLa (human), NIH 3T3 (mouse) Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: F4514-70B
Reactivities: Human
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 200ul
Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Serum.
References: 1. James,D.I., Parone,P.A., Mattenberger,Y. and Martinou,J.C. hFis1, a novel component of the mammalian mitochondrial fission machinery J. Biol. Chem. 278 (38), 36373-36379 (2003). 2. Dohm,J.A., Lee,S.J., Hardwick,J.M., Hill,R.B. and Gittis,A.G. Cytosolic domain of the human mitochondrial fission protein fis1 adopts a TPR fold. Proteins 54 (1), 153-156 (2004). 3. Yu et.al Journal of Cell Science 118, 4141-4151 (2005) 4. Karren et.al JCB, 171 (2), 291-301 (2005) 5. Koch et.al MCB, 16 (11); 5077-5086 (November 2005).
Additional info: Recognizes human Fis1. Species sequence homology: mouse, rat, chimpanzee, canine.

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