FOXM1 (Forkhead Box M1, FKHL16, HNF-3 Forkhead Homolog 11, HFH11, Winged Helix Factor from INS-1 Cells, M-phase Phosphoprotein 2, MPM-2 Reactive Phosphoprotein 2, Transcription Factor Trident)

Forkhead box M1 (FoxM1) is a forkhead box family transcription factor that regulates a number of genes throughout the cell cycle to help control DNA replication, mitosis and cell proliferation. FoxM1 expression increases during G1 and S and reaches maximum levels in G2/M (1-3). Nuclear translocation occurs just before entry into G2/M and is associated with FoxM1 phosphorylation (4). Phosphorylation of FoxM1 by MAPK (Ser331 Ser704), Cyclin/Cdk (Thr600, Thr611, Thr672), Plk1 (Ser715, Ser724) and Chk2 (Ser376) stabilizes and activates FoxM1 (4-7). Forkhead box M1 is expressed in all embryonic tissues but is restricted to proliferating tissues in adults (8). Recent studies have shown that FoxM1 expression is negatively regulated by p53 (9,10). Upregulation of FoxM1 is associated with many human cancers, including prostate, breast, lung, ovary, colon, pancreas, stomach, bladder, liver and kidney, and may be associated with p53 mutations in some tumors (10,11). As a result, FoxM1 inhibitors have become a topic of interest for potential cancer therapy (12). \n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% \n Tween-20 at 4 degrees C with gentle shaking, overnight. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F9048-25C

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, NHP

Form

Supplied in 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Wang, I.C. et al. (2005) Mol Cell Biol 25, 10875-94, 2. Leung, T.W. et al. (2001) FEBS Lett 507, 59-66, 3. Wang, X. et al. (2002) Proc Natl Acad Sci USA 99, 16881-6, 4. Ma, R.Y. et al. (2005) J Cell Sci 118, 795-806, 5. Laoukili, J. et al. (2008) Mol Cell Biol 28, 3076-87, 6. Fu, Z. et al. (2008) Nat Cell Biol 10, 1076-82, 7. Tan, Y. et al. (2007) Mol Cell Biol 27, 1007-16, 8. Ye, H. et al. (1997) Mol Cell Biol 17, 1626-41, 9. Barsotti, A.M. and Prives, C. (2009) Oncogene, Epub ahead of print, 10. Pandit, B. et al. (2009) Cell Cycle 8, 3425-7, 11. Pilarsky, C. et al. (2004) Neoplasia 6, 744-50, 12. Gartel, A.L. (2008) Expert Opin Ther Targets 12, 663-5.

Additional Info

Recognizes human FoxM1. Species crossreactivity: monkey