FOXO1a (FKH1, FKHR, FOXO1, Forkhead Box O1a, Forkhead Homolog1, Forkhead Rhabdomysarcoma)

FKHR (ForKHead in Rhabdomyosarcoma), a 70kD protein, is one member in a subfamily of the forkhead homeotic gene family of transcription factors. Recent experiments have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the carboxyl terminal region of the FKHR protein.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F9049-02C

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum

Form

As reported

P Type

Mab

Purity

Mouse ascites

Isotype

IgG1,k

References

1.Galili, N., et al. (1993) Fusion of a fork head domain gene to PAX3 in the solid tumour alveolar rhabdomyosarcoma. Natl Genet 5:230-235.\n2.Davis, R.J., et al. (1995) Structural characterization of the FKHR gene and its rearrangement in alveolar rhabdomyosarcoma. Hum Mol Genet 4:2355-2362. \n3.Anderson, M.J., et al. (1998) Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics 47:187-199.\n4.Biggs, et al. (1999) Protein kinase B/Akt-mediated phosphorylation promotes nuclear exclusion of the winged helix transcription factor FKHR1. Proc Natl Acad Sci USA 96:7421-7426. \n5.Tang, E.D et al. (1999) Negative regulation of the forkhead transcription factor FKHR by Akt. J Biol Chem 274:16741-16746. \n6. Rena, G., et al. Phosphorylation of the transcription factor forkhead family of the FKHR gene and its rearrangment in alveolar rhabdomyosarcoma. Hum Mol. Genet. 4:2355-2362, 1995.\n7. Brunet, A., et al. Akt promotes cell survival by phosphorylating and inhibiting a forkhead transcription factor. Cell. 96:857-868, 1999.

Additional Info

Recognizes human FKHR (ForKHead in Rhabdomyosarcoma), a 70kD protein.

Alternative Names

DAF-16, Forkhead, Drosophila, Homolog of, in Rhabdomyosarcoma