FOXO3A (FOXO3A, AF6q21, FKHRL1, FKHRL1P2, forkhead box O3A, forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1)

Alternate Name: FOXO3A, AF6q21, FKHRL1, FKHRL1P2, forkhead box O3A, forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1\n\nDomain Information: cd00059: Forkhead (FH), also known as a 'winged helix'\n\nGene Ontology Terms: transcription factor activity; induction of apoptosis; cell growth and/or maintenance; regulation of transcription, DNA-dependent; apoptosis; transcription from Pol II promoter; cytoplasm; nucleus\n\nApplication(s):\nPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 95kD band observed in Human Brain and Heart lysates (predicted MW of 77kD according to NP_001446). Recommended for use at 0.5-2 ug/ml.\n\nStaining (0.5ug/ml) of Human Brain lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See F9049-30Q-P. Antibody

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SPECIFICATIONS

Catalog Number

F9049-30Q

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

Brunet A, Bonni A, Zigmond MJ, Lin MZ, Juo P, Hu LS, Anderson MJ, Arden KC, Blenis J, Greenberg ME. , Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor., Cell. 1999 Mar 19;96(6):857-68.

Additional Info

Recognizes Human, Mouse, Rat, canine FKHRL1/FOXO3A