FOXP3 (Forkhead Box P3, AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID) (FITC)

One of the many immunotolerance mechanisms that the immune system has developed to distinguish between self and non-self antigens is regulatory T cells or Tregs. Several elegant experiments using transgenic mice and retrovirus-mediated over expression studies, have led to the identification of FOXP3, a trascription factor, as a specific molecular marker essential for the development and function of Tregs. The primary evidence regarding the involvement of FOXP3 in the development of Tregs was provided in patients suffering from IPEX, a rare and fatal human autoimmune disease. The emergence of Tregs and the role of FOXP3 as a critical player in the negative control of various normal and pathological immune responses holds great promise for the development of novel therapies for autoimmune diseases.\n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry (Intracellular): 10ul labels1x10e6 cells\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHuman PBMC, mouse splenocytes\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

F9050-10M

Size

1ml

Applications

FC

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 10mM Tris, 150mM sodium chloride, 0.05% BSA, 0.05% sodium azide. Labeled with FITC.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Marc A. Gavin, Troy R. Torgerson, Evan Houston, Paul deRoos, William Y. Ho, Stray-Pedersen, Elizabeth L. Ocheltree, Philip D. Greenberg, Hans D. Ochs, and Alexander Y. Rudensky. Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development. Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6659-64. Epub 2006 Apr 14.

Additional Info

Recognizes mouse FOXP3. Species Crossreactivity: human.

Alternative Names

Scurfin