HDAC11 (Histone Deacetylase 11, HD11, HD-11, FLJ22237)

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins. Eight members of HDAC family have been identified in the past several years. These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in aa sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns. Recently, a new member of this family, HDAC-11, has been cloned by Gao et al. HDAC-11 transcripts were limited to kidney, heart, brain, skeletal muscle, and testis. These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman, mouse, or rat brain\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1827-57L

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Gao L, Cueto MA, Asselbergs F, and Atadja P. J. Biol. Chem. 28: 25748-25755 (2002). 2. Meinke PT and Liberator P. Curr Med Chem, 8(2):211-235 (2001). 3. Nakayama T and Takami Y. J Biochem (Tokyo) 2001, 129 (4):491-499 (20001). 4. Cress, W.D. and Seto, E. J. Cell. Physiol. 184, 1-16 (2000).

Additional Info

Recognizes human HDAC-11. Species Crossreactivity: mouse and rat. Species sequence homology: 100% in multiple HDAC11 isoforms.