HDAC6, Recombinant, Human (Histone Deacetylase 6, FLJ16239, HD6, JM21, KIAA0901)

Full length, recombinant, human Histone Deacetylase 6 with N-terminal GST tag expressed in baculovirus expression system.\n\nMolecular Weight: 159kD, expressed in baculovirus expression system.\n\nApplications: Suitable for use in enzyme kinetics, screening inhibitors and selectivity profiling.\n\nSpecific Activity: \n~50U/ug\n\nUnit Definition:\nOne U is equal to 1 pmol/min\n\nAssay Condition: \n25mM Tris/HCl, pH 8.0, 137mM sodium chloride, 2.7mM KCl, 1mM MgCl2, 0.1mg/ml BSA, 30uM HDAC substrate, H1827-66B. \nIncubation: 30 min. at 37C\n\nStorage and Stability: \nFor long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.

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SPECIFICATIONS

Catalog Number

H1827-66B

Size

50ug

Applications

ICC, IHC, WB

Form

Supplied as a liquid in 25mM Tris HCl, pH 8.0, 138mM sodium chloride, 0.05% Tween-20, 20mM gluthione, 10% glycerol.

Purity

~ 30% by SDS-PAGE

References

1. Roles of histone acetyltransferases and deacetylases in gene regulation: M.H. Kuo & C.D. Allis; Bioessays 20, 615 (1998) 2. Cloning and characterization of the mouse histone deacetylase-2 gene: Y. Zeng, et al.; J. Biol. Chem. 273, 28921 (1998) 3. Ligand-induced recruitment of a histone deacetylase in the negative feedback regulation of the thyrotropin beta gene: S. Sasaki, et al.; EMBO J. 18, 5389 (1999)