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HdmX (MDM4, HdmX, MdmX -Hdm4, Homolog of Mouse Double Minute 4 Protein)

Cat no: H1827-70C

HdmX (MDM4, HdmX, MdmX -Hdm4, Homolog of Mouse Double Minute 4 Protein)

HdmX/MDM-4 is the human homolog of the murine double minute 2 (Mdm2) protein. It is a critical negative regulator of the p53 tumor suppressor.\n\nApplications: \nSuitable for use in Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Proximity Ligation Assay and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 0.5ug per 10e6 cells in 150ul total volume\nImmunocytochemistry/Immunofluorescence: 1:250-1:1,000\nImmunohistochemistry (frozen and paraffin): 1:500-1:2,000\nImmunoprecipitation: 2-5ug/mg lysate\nWestern Blot: 1:10,000-1:25,000\nProximity Ligation Assay: 1:1,000-1:10,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1827-70C

Size

100ug

Applications

FC, ICC, IF, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in Tris-citrate/phosphate buffer, pH 7.5, 0.09% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human HdmX/MDM4 protein. Minimal sequence homology with mouse MdmX.

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