HDV Ag, BioAssay(TM) ELISA Kit

What causes Hepatitis D virus is an agent known as Delta agent (a defective 36nm-43nm RNA virus), which puts it into the classification of Hepatitis delta virus. Hepatitis D can only reproduce when it is linked with Hepatitis B virus (HBV). The disease is transmitted through the skin or through sexual contact with infected blood. Generally, the relationship of HDV and HBV involves some of the most acute and chronic HBsAg carriers. Because of this co-infection, it is important to know whether the HDV and HBV developed at the same time, or if the patient was already a carrier of HBsAg. The co-infection, especially super-infected HDV, can develop into severe acute hepatitis disease. Chronically infected HBV patients infected with HDV have a 70-80% chance of developing cirrhosis of the liver. \nSerologically, it is important to know the specific HDV antibodies (anti-HDV) or antigens (HDV-Ag) to give an accurate diagnosis. Six to eight weeks after exposure and co-infection, identifiable concentrations of HDV specific antigens appear. They clear up during the convalescence stage and, together with heightened levels of HDV antibodies, indicate recovery. However, circulating HDV-Ag is a signal that an acute infection is present, and it is present only transiently at very low levels. During super infection with HDV, identifiable levels of HDV antigens are present two weeks after exposure. Chronic, long-term hepatitis develops if, after this two-week period, the infection fails to clear up.\n\nIntended Use:\nThe purpose of the HDV Ag, BioAssay(TM) ELISA Kit is for clinical lab diagnosis of patients who are suspected of having a hepatitis D (HDV-Ag) virus infection, and for epidemic analysis. The test has 100% sensitivity and specificity. The HDV Ag, BioAssay(TM) ELISA Kit is an enzyme-linked immunosorbent assay for the qualitative determination of hepatitis D antigens in human serum/plasma. \n\nSensitivity:\n100%\n\nSpecificity:\n100%\n\nRange:\nQualitative; positive & negative control & cut off\n\nTest Principle:\nThe HDV Ag, BioAssay(TM) ELISA Kit employs the solid phase, two-step incubation double antibody sandwich method. The patient's serum/plasma is added together with extraction solution after the polystyrene microwell strips are pre-coated with purified antibodies specific to HDV. If the HDV virus is present, the HDV particles are disrupted and what's captured in the wells is the specific HDV antigens. At this stage, unbound serum proteins must be washed off the microwells. What is added next is Horseradish Peroxidase (HRP) which is conjugated with a secondary antibody. Again, after washing, unbound conjugates are removed. Added to the wells after this are both the chromogen solutions containing Tetramethylbenzidine (TMB) and urea peroxide. During this stage of a combined presence of antibody-antigen-antibody (HRP) sandwich immunocomplex, a blue-colored product appears, which is the result of colorless chromogens hydrolyzed by the bound HRP conjugate. After stopping the reaction with sulfuric acid, the blue color turns yellow. The color intensity can be gauged proportionally to the amount of antigen in the sample. Colorless wells appear when samples are negative for HDV antigens. \n\nKit Components:\n1. Microwell Plate, 1x96 wells\n2. Negative Control, 1x0.5ml\n3. Positive Control, 1x0.5ml\n4. HRP-conjugate Reagent, 1x12ml\n5. Extraction Solution, 1x6ml\n6. Stock Wash Buffer, 1x50ml\n7. Chromogen Soluation A, 1x7ml\n8. Chromogen Solution B, 1x7ml\n9. Stop Solution, 1x7ml\n10. Sealable Bag, 1unit\n11. Plate Cover, 2 sheets\n\nStorage and Stability:\nStore components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

138777

Size

1Kit

Applications

ELISA

References

1. Purcell, R.H. and Gerin, J.L., Hepatitis Delta virus. In: Fields Virology, 3rd ed. Philadelphia, Lippincott-Raven, 1996. 2. Hadziyannis SJ. Hepatitis delta: an overview. In: Rizzetto M, Purcel RH, Gerin JL, and Verme G,eds. Viral hepatitis and liver disease, Turin, Edizoni Minerva medica, 1997. 3. Lai, M.C.C., The molecular biology of hepatitis Delta virus. Annual Review of Biochemistry 64: 259-286 (1995). 4. Centers for Disease Control and Prevention. Epidemiology and Prevention of Viral Hepatitis A to E: An Overview 2000. 5. Hepatitis Delta: WHO/CDS/CSR/NCS 2001.1