HDV IgG BioAssay(TM) ELISA Kit

What causes Hepatitis D virus is an agent known as Delta agent (a defective 36nm-43nm RNA virus) which puts it into the classification of Hepatitis delta virus. Hepatitis D can only reproduce when it is linked with Hepatitis B virus (HBV). The disease is transmitted through the skin or through sexual contact with infected blood. Generally, the relationship of HDV and HBV involves some of the most acute and chronic HBsAg carriers. Because of this co-infection, it is important to know whether the HDV and HBV developed at the same time, or if the patient was already a carrier of HBsAg. The co-infection, especially super-infected HDV, can develop into severe acute hepatitis disease. Chronically infected HBV patients infected with HDV have a 70-80% chance of developing cirrhosis of the liver. \nSerologically, it is important to know the specific HDV antibodies (anti-HDV) or antigens. Current, or past infection, is shown by anti-HDV IgG antibodies. Chronic or active infections are typically represented by high titers of the antibodies. Through the course of HBV-HDV co-infection, and after the tenth week of exposure, identifiable concentrations of anti-HDV IgG appear. If cleared up during convalescence, then evidence of recovery is shown. Recognizable levels of antibodies appear during HDV superinfection and four to six weeks after exposure. Long term, chronic carrier stage is possible if the infection does not clear up after this stage. \n\nIntended Use:\nThe purpose of the HDV IgG BioAssay(TM) ELISA Kit is for clinical lab diagnosis and treatment of patients who are suspected of having a hepatitis D virus infection. The HDV IgG BioAssay(TM) ELISA Kit is an enzyme-linked immunosorbent assay for qualitative identification of IgG antibodies to hepatitis D virus in human serum/plasma. \n\nSensitivity:\n100%\n\nSpecificity:\n100%\n\nRange:\nQualitative positive; negative control & cut off\n\nTest Principle:\nThe HDV IgG BioAssay(TM) ELISA Kit employs the solid phase, two-step incubation, indirect ELISA method. Recombinant HDV antigens are pre-coated on the polystyrene microwell strips. Anti-HDV specific antibodies, if present, will appear during the first incubation stage - and be bound to the solid phase pre-coated HDV antigens. After this step, the wells are washed to remove unbound serum proteins. Next it is important to add anti-human IgG antibodies (anti-IgG) conjugated to the enzyme horseradish peroxidase (HRP-Conjugate). These HRP-conjugated antibodies, during the second incubation stage, will be bound to any antigen-antibody (IgG) complexes that were formed before. At this point, the unbound HRP-conjugate is then removed by washing. Added to the wells after this are both the chromogen solutions containing Tetramethylbenzidine (TMB) and urea peroxide. During this stage of the presence of the antigen-antibody-anti-IgG (HRP) immunocomplex, a blue-colored product appears, which is the result of colorless chromogens hydrolyzed by the bound HRP conjugate. After stopping the reaction with sulfuric acid, the blue color turns yellow. The color intensity can be gauged proportionally to the amount of antibody captured in the wells, and the amount of antibody in the sample, respectively. Colorless wells appear when samples are negative for HDV IgG. \n\nKit Components:\n1. Microwell plate, 1x96 wells\n2. Negative Control, 1x0.5ml\n3. Positive Control, 1x0.5ml\n4. HRP-conjugate reagent, 1x12ml\n5. Specimen Diluent, 1x12ml\n6. Stock Wash Buffer, 1x50ml \n7. Chromogen Solution A, 1x7ml\n8. Chromogen Solution B, 1x7ml\n9. Stop Solution, 1x7ml\n10. Sealable Bag, 1unit\n11. Plate Cover, 2 sheets\n\nStorage and Stability:\nStore components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

138776

Size

1Kit

Applications

ELISA

References

1. Purcell, R.H. and Gerin, J.L., Hepatitis Delta virus. In: Fields Virology, 3rd ed. Philadelphia, Lippincott-Raven, 1996. 2. Hadziyannis, S.J., Hepatitis delta: an overview. In: Rizzetto M, Purcel RH, Gerin JL, and Verme G,eds. Viral hepatitis and liver disease, Turin, Edizoni Minerva medica, 1997.\n3. Lai, M.C.C., The molecular biology of hepatitis Delta virus. Annual Review of Biochemistry, 64: 259- 286 (1995). 4. Centers for Disease Control and Prevention. Epidemiology and Prevention of Viral Hepatitis A to E: An Overview 2000.