HeLa Cell Lysate, Heat Shocked

HeLa Cell Lysate (Heat Shocked) is useful in immunoblotting applications as a positive control for many antibodies, particularly those recognizing proteins induced by heat shock. The cells are grown, heat shocked for 2 hours at 44 degrees C, and allowed to recover at 37 degrees C for 5 to18 hours before harvest. The cells are then washed, lysed, sonicated, and resuspended in SDS-PAGE sample buffer.\n\nThe HeLa cell line was originally derived from epithelioid carcinoma tissue (cervix) from a human donor. Research into the heat shock response provides a model for the study of the effects of environmental and homeostatic stressors upon cells. Heat shocking reproducibly alters gene expression in cells resulting in the subsequent production of a variety of morphological changes as well as the expression of heat shock proteins which are crucial for the maintenance of cellular homeostasis during normal cell growth and for survival during and after various cellular stresses. Heat shocking has been shown to increase morphological changes compatible with\napoptosis in many cell types. In mammalian cells, the induction of the heat shock response requires the activation and nuclear translocation of transcription factors which control the expression of the cytoprotective heat shock proteins as well as playing pivotal roles in the development of inflammation and immunity.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 20ug/lane\nOptimal dilutions to be determined by the researcher.\n\nProduction Method:\nHeLa cells were maintained in 90% Eagle

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SPECIFICATIONS

Catalog Number

H1838-01

Size

200ug

Form

Supplied as a liquid in SDS PAGE sample buffer (40mM Tris-HCl, pH 8.8, 1% SDS, 50mM DTT, 7.5% glycerol, 0.003% bromphenol blue), heated for 10 minutes at 70C.

References

1. Morita-Fujimura, Y., Fujimura, M., Yoshimoto, T., Chan, P.H. (2001) Stroke 32(10):2356-2361. 2. Patel, H.H., Hsu, A., Gross, G.J. (2002) Am J Physiol 282(6): H2011-H2017.