Hepcidin, Mouse, 13aa, CT, Control Peptide (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide)

Control Peptide for H2008-53 (antiserum) and H2008-54 (affinity purified antibody).\n\nHepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, proprotein in mouse 83 aa, rat/human 84 aa) are almost exclusively produced in liver. Human hepcidin is produced from 84-aa precursor, including a putative 24-aa signal peptide. The secreted form of hepcidin in blood and urine is consists of C-terminal 20, 22 or 25-aa residues. The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice. The human gene is located at chromosome 19, in close proximity with Usf2 gene. Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice. Hepcidins are devoid of IRE. Like other antimicrobial peptides, hepcidin is upregulated by lipopolysaccharides (LPS).\n\nApplications: \nSuitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 50-100ng control peptide/well.\nAntibody Blocking: 5-10ug per 1ul H2008-53 (antiserum) or per 1ug H2008-54 (affinity purified antibody).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H2008-55

Size

50ug

Applications

ELISA, WB

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

Purity

Highly purified

References

1. Pigeon, C., et al. (2001) JBC 276, 7811-7819. 2. Park, C.H., et al. (2001) JBC 276, 7806-7810. 3. Krause, A., et al. (2000) FEBS Lett. 480, 147-150. 4. Fleming, R.E. and Sly, W.S. (2001) PNAS 99, 8160-8162. 5. Nicolas, G., et al. (2002) PNAS 99, 4596-4601.

Additional Info

Synthetic peptide 13aa sequence within the C-terminus of mature, 25aa mouse HEPC. Species Sequence Homology: Rat: 92%; Human: 69% (20-, 22- and 25-aa C-terminal fragments).