Heterochromatin Protein 1, alpha (HP1a)

Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. \nHeterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1a, HP1b and HP1g). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.\n\nApplications: \nSuitable for use in ELISA, Immunohistochemistry and Immunocytochemistry. Not suitable for Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:500-1:5000\nImmunohistochemistry: 1:500-1:5000\nImmunocytochemistry: 1:500-1:5000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHeLa cells, 3T3 cells, F9 cells, mouse embryos\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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