ATP: D-hexose 6-phosphotransferase (EC 2.7.1.1) from yeast
Specific Activity: (same/more than)140u/mg protein
Reaction Equation:
ATP + D-Hexose = ADP + D-Hexose-6-phosphate
Contaminants:
Phosphoglucose isomerase (same/less than) 0.1%
Glutathione reductase (same/less than) 0.05%
Myokinase (same/less than) 0.01%
Phosphogluconate dehydrogenase (same/less than) 0.005%
Phosphoglucomutase (same/less than) 0.01%
Glucose-6-phosphate dehydrogenase (same/less than) 0.01%
ATPase (same/less than) 0.001%
Creatine kinase (same/less than) 0.005%
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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