HGF (Hepatocyte Growth Factor, Hepatopoietin-A, Scatter Factor, SF) (Biotin)

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity. Canine HGF is secreted as an inactive 699aa single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide linked HGF with a 60kD A and 30kD B chain. A variant of HGF with a 5aa deletion in the first Kringle domain has been described in canine and other species. Canine HGF shares 97%, 99%, 93%, 93%, and 89% aa sequence identity with bovine, feline, human, mouse, and rat HGF, respectively. HGF binds heparan sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGR/cMET. HGF dependent cMET activation is implicated in the development of many human cancers. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. HGF induces the upregulation of integrin A2B1 in epithelial cells by a selective increase in A2 gene transcription. This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis. HGF can also alter epithelium morphology by the induction of nectin-1a ectodomain shedding, an adhesion protein component of adherens junctions. In the thymus, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSHs-timulated iodine uptake. HGF promotes the motility of cardiac stem cells in damaged myocardium.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1mg/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile Tris, pH 7.3 (20mM Trizma base, 150mM sodium chloride), 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H2005-08D

Size

50ug

Applications

WB

Hosts

Sheep

Reactivities

Hum, Mouse, Can

Form

Supplied as a lyophilized powder in PBS, BSA. Reconstitute with sterile Tris, pH 7.3 (20mM Trizma base, 150mM sodium chloride), 0.1% BSA to 0.2mg/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40. 2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21. 3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3. 4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501. 5. Miyake, M. et al. (2003) Vet. Immunol. Immunopathol. 95:135. 6. Mizuno, K. et al. (1994) J. Biol. Chem. 269:1131. 7. Neo, S. et al. (2005) J. Vet. Med. Sci. 67:525. 8. Corso, S. et al. (2005) Trends Mol. Med. 11:284. 9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.10. Montesano, R. et al. (1991) Cell 67:901.11. Chiu, SJ. et al. (2002) J. Biomed. Sci. 9:261. 12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531. 13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472. 14. Dremier, S. et al. (1994) Endocrinology 135:135. 15. Linke, A. et al. (2005) Proc. Natl. Acad. Sci. 102:8966.

Additional Info

Recognizes canine HGF. Species Crossreactivity: Recombinant human (50%), recombinant mouse (15%), rhMSP (<3%).