HIF3A (MOP7, IPAS, , PASD7, bHLHe17, HIF3-alpha-1, HIF-3-alpha, HIF3-alpha, Inhibitory PAS domain protein, Member of PAS protein 7, PAS domain-containing protein 7, Basic-helix-loop-helix-PAS protein MOP7, Class E basic helix-loop-helix protein 17)

Hypoxia-inducible factor 3 alpha, also called MOP7, is a protein that in humans is encoded by the HIF3A gene. HIF3A is mapped to 19q13.32. This gene involved in adaptive response to hypoxia. HIF3A suppresses hypoxia-inducible expression of HIF1A and EPAS1. It binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. This gene functions as an inhibitor of angiogenesis in the cornea.\n\nUniProt Number:\nQ9Y2N7\n\nGene ID:\nHIF3A\n\nApplications:\nSuitable for use in Western Blot.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144664

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Hara, S., Hamada, J., Kobayashi, C., Kondo, Y., Imura, N. Expression and characterization of hypoxia-inducible factor (HIF)-3-alpha in human kidney: suppression of HIF-mediated gene expression by HIF-3-alpha. Biochem. Biophys. Res. Commun. 287: 808-813, 2001.\n2. Maynard, M. A., Qi, H., Chung, J., Lee, E. H. L., Kondo, Y., Hara, S., Conaway, R. C., Conaway, J. W., Ohh, M. Multiple splice variants of the human HIF-3-alpha locus are targets of the von Hippel-Lindau E3 ubiquitin ligase complex. J. Biol. Chem. 278: 11032-11040, 2003.\n

Additional Info

Recognizes human and rat HIF3A. No crossreactivity with other proteins.