His-Tag (6-Histidine)

The His-tagged expression system is widely used in the production of recombinant protein. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by single-step chromatography. The His-tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.\n\nApplications:\nSuitable for use in Immunoprecipitation, ELISA and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nImmunoprecipitation: 10ug/application\nELISA: 0.1-1ug/ml\nWestern Blot: 1-5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H5099-05A

Size

100ug

Applications

ELISA, IP, WB

Hosts

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, containing 0.1% sodium azide

P Type

Mab

Purity

Purified by Protein A chromatography

Isotype

IgG1,k

Additional Info

Specific for 6xHis. On Western blots, it identifies a band at ~ 37kDa. Reactivity has been confirmed with TS-His fusion protein, and is expected to react with all His-tagged proteins regardless of species.