HLA Class 1 Antigen A2,A24

Applications:\nThis antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies.\n\nRecommended Dilution:\nFor a cytotoxic working dilution, add 0.9ml of PBS, 1% BSA to 100ul of10X stock solution.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. May be stored at 4 degrees C for short-term only. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nNote: FITC-labeled and purified versions of these antibodies are available on special order.\n\nPlease contact United States Biological for bulk requirements and licensing requests relating to production applications.

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SPECIFICATIONS

Catalog Number

H6098-11

Size

100ul

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, 1% BSA. Reconstitute to 100ul sterile dH2O to make a 10X stock solution.

P Type

Mab

Purity

Ascites

Isotype

IgM

References

1. NCCLS Tentative Standard. \"Leukocyte Differential Counting\" Publication Number H20-T, NCCLS, Vol. 4, No. 11 (1984). 2. Pei, R., et al., Amonospecific HLA-27 fluorescein isothiocyanate conjugated monoclonal antibody for rapid, simple and accurate HLA-B27 typing. Tissue Antigen 41: 200-203 (1993). 3. Pei, R., Woo, G. and Lee, J.H. \"Detection of blood chimerism at a frequency of one per thousand by flow cytometry. Visuals of the clinical histocompatibility workshop\", Paul I. Terasaki, Ed., pp.73-74 (1995). 4. Pei, R., Chen, T., Orpilla, J. and Lee, J.H. \"A simultaneous negative and positive selection method that can detect chimerism at a frequency of 1 per 10,000 by flow cytometry\", Tissue Antigens 50:197-201 (1997).

Additional Info

Reacts to HLA Class 1 Antigen-A2,A24.