IgA, Secretory Component (sIgA, Secretory IgA) (FITC)

Secretory component is present in human secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. Under normal conditions, sIgA contains both subclasses IgA1 and IgA2, since both are capable of binding SC. SC also has an affinity for polymeric IgM.\n\nApplications: \nSuitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nFluorescein/IgG protein molar ratio (F/P): ~1.6\n\nExcitation: 492nm\n\nEmission: 515nm\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I1890-31F

Size

1ml

Applications

ELISA

Hosts

Goat

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.2. Preservative free. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.

P Type

Pab

Purity

Purified by DEAE chromatography

Isotype

IgG

Additional Info

Recognizes human Secretory Component. Reacts with both bound secretory component (secretory IgA) and with the free SC present in human secretions. In immunoelectrophoresis against human milk, using a high electroendosmosis agar plate, free SC is precipitated in the alpha-2 region. Does not react with other molecular forms of IgA, or with any other secretory or plasma protein.