Influenza A, Texas 1/77 (H3N2)

Influenza virus is a single-stranded RNA virus, segmented, 70nm diameter and enveloped. Strains are described by geographic origin, strain number, year of isolation and hemagglutination (H) and neuraminidase (N) antigens. It is an acute viral disease of the uper respiratory tract characterized by fever, headache, chills, myalgia, weakness, runny nose and sore throat. Cough can be severe. Nausea and vomiting are uncommon. Fatality is generally low, except for those with chronic lung or heart conditions.\n\nSource: \nInfluenza A antigen, Texas strain, produced in embryonated chicken eggs. \n\nPurification: \nEggs are infected by the amniotic route, incubated for 72 hours and refrigerated overnight. Amniotic fluids are harvested from live eggs and the embryos are washed in PBS. The suspension was concentrated using cross-flow ultracentrifugation system with 30,000 molecular weight cut off. The concentrate is sonicated and clarified using low speed centrifugation. This preparation contains a high concentration of viral antigens as well as some egg proteins in PBS.\n\nConcentration: ~0.60mg/ml (dye binding assay).\nHA Titer: 73,000 HA units/ml\n\nInactivation:\nGamma radiation inactivation. Effectiveness determined when no detection of growth in two blind passages under the original culture conditions. Result: No live virus detected by CPE or HA assay.\n \nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution: \nThis antigen preparation should be sonicated with a microtip immediately prior to use to ensure that the preparation is uniform. A precipitate may form upon thawing, due to high product concentration. Dilute prior to clarification or other manipulation. Optimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts