Interleukin 1 Receptor AcP, C2 (Interleukin-1 Receptor Accessory Protein, IL-1 Receptor Accessory Protein, IL-1RAcP, IL1RAP, C3orf13, FLJ37788, Interleukin-1 Receptor 3, IL1R3, IL-1R3, IL-1R-3)

The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP) (1). IL-1RAcP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associated kinase (IRAK), which mediates activation of NF-kB inducing kinae (NIK) and of NF-kB, recruits to the IL-1R complex through IL-1RAcP. IL-1 activation of stress-activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:2000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa whole cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

I7663-25Y1

Size

50ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Greenfeder SA, Nunes P, Kwee L, Labow M, Chizzonite RA, Ju G. Molecular cloning and characterization of a second subunit of the interleukin 1 receptor complex. J Biol Chem 1995;270:13757-65. 2. Huang J, Gao X, Li S, Cao Z. Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin 1 receptor accessory protein. Proc Natl Acad Sci USA 1997;94:12829-32. 3. Wesche H, Korherr C, Kracht M, Falk W, Resch K, Martin MU. The interleukin-1 receptor accessory protein (IL-1RAcP) is essential for IL-1-induced activation of interleukin-1 receptor-associated kinase (IRAK) and stress-activated protein kinases (SAP kinases). J Biol Chem 1997;272:7727-31. 4. Hofmeister R, Wiegmann K, Korherr C, Bernardo K, Kronke M, Falk W. Activation of acid sphingomyelinase by interleukin-1 (IL-1) requires the IL-1 receptor accessory protein. J Biol Chem 1997;272:27730-6.

Additional Info

Recognizes human IL-1RAcP (C2).