Interleukin 2 (IL-2)

Human interleukin-2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene.\n \nApplications:\nSuitable for use in RIA, Western Blot, Immunoprecipitation, and neutralization procedures. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10\nFor neutralization of biological activity in vitro dilutions have to be made according to the amount of IL-2 to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

I7663-26C1

Size

100ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

Additional Info

Recognizes human Interleukin-2 (Il-2) and inhibits the biological activity of human IL-2 and lacks reactivity with rat and mouse IL-2. The antibody shows no detectable reactivity in RIA with human Interleukin-1, TNF-alpha and Interferon gamma.