Kallikrein 11, Recombinant, Human (KLK11, Hippostasin, Trypsin-like serine protease and PRSS20)

Kallikrein 11 is expressed in brain, skin, ovary and prostate. Increased levels of Kallikrein 11 have been reported in prostate and ovarian carcinoma. It is a serine protease. Kallikrein 11 (KLK11), also known as hippostasin, trypsin-like serine protease and PRSS20, is a member of human tissue kallikrein family.6 Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences, respectively.1-4. Isoform 1 consists of a signal peptide (residues 1-18), a short pro peptide (residues 19-21) and the mature chain\n(residues 22-250). Isoform 2 is identical to isoform 1, except that a 32 amino acid segment is inserted in isoform 2 before residue1 in isoform 1. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate. KLK11 is a novel marker for ovarian and prostate cancer carcinomas.7-9 rhKLK11, after being activated by thermolysin, is active against a thioester substrate. rhKLK11 corresponds to isoform 1.\n\nSource: Human CD33 Signal Peptide (Met 1-Ala 16) Human Kallikrein 11 (Glu 19-Asn 250) HHHHHHHHHHNC\n\nRecombinant human Kallikrein 11 (rhKLK11, residues 19-250)1-4 in the above construct was\nexpressed in a murine myeloma cell line, NS0.\n\nMolecular Mass: The purified, secreted rhKLK11 corresponds to the pro form with an N-terminal sequence of E19TRIIKGFE. By SDS-PAGE under reducing conditions, the apparent molecular mass of the pro form is approximately 40kD.\n\nPurity: (same/more than)95%, as determined by SDS-PAGE and visualized by silver stain.\n\nEndotoxin: (same/less than) 1EU per 1ug of the protein as determined by the LAL method.\n\nActivity: Measured by its activity to cleave a colormetric peptide substrate D-Val-Leu-Lys-ThioBenzyl ester (VLK-SBzl), in the presence of 5,5

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SPECIFICATIONS

Catalog Number

K0005-14D

Size

10ug

References

1. Yoshida, S. et al., 1998, Biochim. Biophys. Acta 1399:225 - 228.\n2. Yousef, G.M. et al., 2000,Genomics 63:88 - 96.\n3. Mitsui, S. et al., 2000, Biochem. Biophys. Res. Commun. 272:205 - 211.\n4. Gan, L. et al., 2000, Gene 257:119 - 130.\n5. Edwards, K.M. et al., 1999, J. Biol. Chem. 274:30486 - 30473.\n6. Yousef, G.M. and E.P. Diamandis, 2001, Endocrine Rev. 22:184 - 204.\n7. Diamandis, E.P. et al., 2002, Cancer Res. 62:295 - 300.\n8. Nakamura, T. et al., 2003, Urology 61:1042 - 1046.\n9. Borgono, C.A. et al., 2003, Int. J. Cancer 106:605 - 610.