MEK1, MEK2, phosphorylated (Ser218/222) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1 / MAPK/ERK Kinase 2, Dual Specificity Mitogen-activat

MEK 1 and MEK 2 are integral components of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activation of MEK 1/2 occurs via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK 1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK. This phosphorylation of ERK by MEK 1/2 is a critical step in the MAP kinase cascade. \n\nRecommended Dilution: \nDot blots: 1:1000\nWestern blots: 1:1000\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2865-03C

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Xen/Amph

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by sequential chromatography on a Protein A column and phospho- and non-phospho-peptide affinity columns.

Isotype

IgG

References

1. N.G. Ahn, N.G., Robbins, D.G., Haycock, J.W., Seger, R., Cobb, M.H. and Krebs, E.G., J. Neurochem. 59 (1992) 147 - 156. \n2. Crews, C.M., Alessandrini, A. and Erikson, R.L., Science 258 (1992) 478 - 480. \n3. Ahn, N.G. Mol. Cell Biochem. 127 - 128 (1993) 201 - 209. \n4. Adams, J.P. and Sweatt, J.D., Annu. Rev. Pharmacol. Toxicol. 42 (2002) 135 - 163.

Additional Info

Specific for MEK 1/2 phosphorylated at Ser218 and Ser222. Species reactivity: human, mouse, rat, and Xenopus.

Alternative Names

EC=2.7.12.2, Protein Kinase Mitogen Activated Kinase 1, Protein Kinase Mitogen Activated Kinase 2