MPM2, Ser, Thr, Pro, phosphorylated (FSE)

Upon entry into M phase, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). The MPM2 monoclonal antibody binds to a phospho amino acid-containing epitope (peptides containing LTPLK and FTPLQ domains) present on more than 50 proteins of M-phase eukaryotic cells.\n\nApplications: \nSuitable for use in Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunocytochemistry: 2-15ug/ml showed positive immunostaining for phosphoproteins in A431 mitotic cells fixed with 3.7% paraformaldehyde.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FSE conjugates are sensitive to light.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

M4685-08

Size

200ug

Applications

ICC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 20mM TBS, pH 8.0, 0.05% sodium azide, 30% glycerol. Labeled with fluorescein-5-EX, succinimidyl ester (FSE).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Davis, F.M., et al., Proc. Natl. Acad Sci. USA 80: 2926-2930 (1983). 2. Harper, J.D.I., et al., Eur. J. Cell Biol. 51: 272-278 (1990). 3. Vandre, D.D., et al., Proc. Natl. Acad. Sci. USA 81: 4439-4443 (1984). 4. Kuang, J., et al., Proc. Natl. Acad. Sci. USA 86: 4982-4986 (1989). 5. Kuang, J., et al., Mol. Biol. Cell 5: 135-145 (1994). 6. Westendorf, J.M., et al, Proc. Natl. Acad. Sci. USA 91: 714-718 (1994). 7. Yaffe, M.B., et al, Science 278: 1957-1959 (1997).

Additional Info

Recognizes a phosphorylated epitope (LTPLK or FTPLQ) in a distinct class of phosphoproteins including MAP2, HSP70, cdc25 and DNA topoisomerase IIa, most of which are phosphorylated at the onset of mitosis. The number of phosphoproteins recognized by MPM-2 varies from species to species and with the type of cell. Species Crossreactivity: Recognizes both animals and plants.