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Multiple Tag Fusion Protein, Positive Control

Cat no: M3889-51

Multiple Tag Fusion Protein, Positive Control

Multiple Tag is a specially designed fusion protein for use as a positive control for Western blot or Dot blot. The fusion protein is a 40kD protein containing 16 commonly used tags from N-terminus to C-terminus in the order as shown in the table:\n\nTag Sequence\nTrx MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY \n QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL\n KEFLDANLA\nT7 CMASMTGGQQMG\nHSV QPELAPEDPEDC\nc-Myc EQKLISEEDLC\nVSV-g YTDIEMNRLGK \nGlu-Glu CEEEEYMPME\nV5 GKPIPNPLLGLDST\nE-tag GAPVPYPDPLEPR\nFlag-tag DYKDDDDK\nS-tag KETAAAKFERQHMDS\nHA YPYDVPDYAC\nKT3 KPPTPPPEPET\nE2 GVSSTSSDFRDR\nAU1 DTYRYI\nAU5 TDFYLK\n6x His HHHHHH \n\nApplications: \nSuitable for use in Western Blot and Dot Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.04-0.2ul per lane of minigel. For accurate loading, dilute with PBS.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nStore lyophilized powder at -20 degrees C or lower. Reconstitute by adding sterile dH2O. Aliquot reconstituted product to avoid repeated freezing and thawing, and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M3889-51

Size

200ug

Applications

WB

Form

Supplied as a lyophilized powder. Reconstitute with 200ul of ddH2O.

Purity

>95% by SDS-PAGE and Coomassie blute staining. Purified from E. coli cell lysate.

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