Multiplex Human Cytokine, BioAssay(TM) ELISA Kit

Multiplex ELISA kit for pro-inflammatory cytokines is designed for semi-quantitative and simultaneous determination of pro-inflammatory cytokines including interleukin 1alpha (IL-1alpha), interleukin 1 beta (IL-1beta), interleukin 6 (IL-6), interleukin 8 (IL-8), granulocyte macrophage colony stimulating factor (GM-CSF), monocyte chemotactic and activating factor (MCAF), and tumor necrosis factor alpha (TNF- alpha) in cell culture supernatant and other biological samples. \n\nThis ELISA assay is a 3.5 hour solid phase immunoassay readily applicable to measure the levels of eight cytokines in cell culture supernatant, and other biological fluids. It showed no cross reactivity with various other proteins. This Multiplex ELISA is expected to be effectively used for investigations into the relationship inflammatory cytokine expression and various experimental, pathological and physiological conditions.\n\nThe kit is intended FOR LABORATORY RESEARCH USE ONLY and is not for use in diagnostic or therapeutic procedures.\n\nAssay Time:\n3.5 hours\n\nKit Components:\n1. ANTIBODY COATED MICROTITER PLATE, The plate contains twelve 8-well ELISA strips. \n2. BIOTIN CONJUGATE MIXTURE (6 ml), Mixture of Biotin conjugated anti-human cytokine antibodies.\n3. HRP CONJUGATE MIXTURE (11ml), Mixture of Horseradish Peroxidase Conjugates\n4. STANDARD MIXTURE (2 Vials), Two vials of lyophilized standard mixture. Each vial contains a buffered protein base and eight pro-inflammatory cytokines at different concentrations: IL-1alpha 1300pg, IL1beta 850pg, IL-6 1000pg, IL-8 2800pg, GM-CSF 1200pg, IFN-g 2000pg, MCAF 1400pg, TNF- alpha 2800pg.\n5. CALIBRATOR DILUENT I (25ml), Newborn calf serum with PBS buffer and preservative. For serum/plasma testing.\n6. CALIBRATOR DILUENT II (25ml), Cell culture medium RPMI 1640 with newborn calf serum and preservative. For cell culture supernatant testing.\n7. WASH BUFFER (20X) (60ml), 20-fold concentrated solution of buffered surfactant.\n8. SUBSTRATE A (10ml), Buffered solution with H2O2\n9. SUBSTRATE B (10ml), Buffered solution with TMB.\n\nStorage and Stability:\nStore kit components at 4 degrees C. Unopened kit components are stable for 6 months at 4 degrees C. Reconstituted standards are stable for up to 30 days when stored sealed at 4 degrees C.

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SPECIFICATIONS

Catalog Number

I8432-08

Size

96Tests

Applications

ELISA

Reactivities

Hum

References

1. CA Dinarello, Proinflammatory Cytokines, Chest 2000:118; 503-508 I Klusman and ME 2. Schwab, Effects of pro-inflammatory cytokines in experimental spinal cord injury Brain research 1997:762(1-2); 173-184. 3. JH Curfs, JF Meis and JA Hoogkamp-Korstanje, A primer on cytokines: sources, receptors, effects, and inducers, Clinical Microbiology Reviews 1997: 10(4); 742-780. 4. J Vilcek and M, Feldmann, Historical review: Cytokines as the therapeutics and targets of the therapeutics, Trends in pharmacological sciences 2004: 25(4); 201-209. 5. M Kapoor, J Martel-Pelletier, D Lajeunesse, JP Pelletier & H Fahmi Role of proinflammatory cytokines in the pathophysiology of osteoarthritis, Nature Reviews Rheumatology 2011:7, 33-42