Mumps IgM, BioAssay(TM) ELISA Kit

Mumps IgM, BioAssay(TM) ELISA Kit is intended for the qualitative detection of IgM antibody in human serum to Mumps for the determination of immunological experience. \n\nThe mumps virus is a member of the paramyxovirus group and the etiological agent of mumps in man. Mumps is a generalized illness usually accompanied by parotid (salivary gland) swelling and mild symptoms. It is also one of the most common causes of aseptic meningitis, encephalitis, and inflammation of the testes (orchitis), pancreas, and ovaries. Parotitis as a presenting symptom in mumps infections is usually sufficiently diagnostic to preclude serological confirmation. However, a third of mumps infections are subclinical or unrecognized (1) and may require viral isolation and/or some other serological procedure to confirm or \nrule out mumps infection. An example of this is presenting orchitis or meningoencephalitis, the \ntwo most common complications of mumps infection, without salivary gland involvement. Virus isolation is time consuming and cumbersome and is usually an impractical procedure for \nthe typical clinical laboratory. Current methods for serodiagnosis of mumps infections are in-vitro serum neutralization, hemagglutination-inhibition (HAI), indirect immunofluorescence, and complement fixation (CF) tests. Of these methods, neutralization is reportedly the most specific. However, the neutralization test requires 4-5 days to complete the test. HAI and CF are reportedly less sensitive than the neutralization test. \n\nThese methods lack specificity, which limits their usefulness in determining immune status. The HAI test also requires pretreatment of test serum to remove nonspecific hemagglutination inhibitors from some serum. Infection with mumps virus, whether symptomatic or subclinical, is generally thought to offer lifelong immunity. Anti-Mumps virus IgM appear 2-3 days after the occurrence of the first clinical symptoms (these remain 2-3 months), followed by the production of Mumps IgG antibodies which persist lifelong. following vaccination with live virus there is a seroconversion in 90% of cases, however, the titre is somewhat lower than in normal infections. As first described by Engvall and Perlmann (2,3,4) and Van Weeman (5), Enzyme Immunoassays can be both specific and sensitive for the detection and measurement of serum proteins. The sensitivity, specificity, and reproducibility of enzyme-linked immunoassays can be comparable to other serological tests for antibody, such as immunofluorescence, complement fixation, hemagglutination and neutralization (6,7,8,9). ELISA is as sensitive as the neutralization test and more sensitive than CF and HAI which makes it a reliable test for determination of immune status. The United States Biological Mumps IgM, BioAssay(TM) ELISA Kiti provides all the necessary reagents for the rapid determination and quantitation of IgM antibody to mumps virus in human serum. \n\nKit Components: \nEach kit contains the following components in sufficient quantities to perform the number of tests indicated. All reactive reagents contain 0.1% sodium azide as a preservative \n\n1. Microtiter Plate\n2. Conjugate\n3. Calibrator\n4. Positive Control\n5. Negative Control\n6. TMB\n7. Wash Buffer, 10X Diluted wash buffer to 1X\n8. Sample Diluent\n9. Stop Solution \n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M9215-08

Size

96Tests

Applications

ELISA

References

1. Kleiman, M. B. 1985. Mumps Virus Infections. In: Laboratory Diagnosis of Viral Infections, E. H. Lennette, ed. Dekker, New York 23: 369-384. 2. Engvall, E., K. Jonsson, and P. Perlmann. 1971. Enzyme-Linked Immunosorbent Assay, (ELISA) Quantitative Assay of Immunoglobulin G. Immunochemistry. 8: 871-874. 3. Engvall, E. and P. Perlmann. 1971. Enzyme-Linked Immunosorbent Assay, ELISA. In: Protides of the Biological Fluids. H. Peeters, ed. Proceedings of the Nineteenth Colloquium, Brugge Oxford. Pergamon Press. pp. 553-556. 4. Engvall, E., K. Jonsson, and P. Perlmann. 1971. Enzyme-Linked Immunosorbent Assay. II. Quantitative Assay of Protein Antigen, Immunoglobulin-G, By Means of Enzyme- Labelled Antigen and Antibody-Coated tubes. Biochem. Biophys. Acta. 251: 427-434. 5. Van Weeman, B. K. and A.H.W.M. Schuurs. 1971. Immunoassay Using Antigen-Enzyme Conjugates. FEBS Letter. 15: 232-235. 6. Bakerman, S. 1980. Enzymed Immunoassays. Lab. Mgmt. August: 21-29. 7. Voller, A., D. Bidwell, and A. Bartlett. 1976. In: Manual of Clinical Immunology, N. Rose, and H. Friedman, eds. pp. 505-512. 8. Voller, A., D. Bidwell, and A. Bartlett. 1976. Bull. Wld. Hlth. Org. 53:55-65. 9. Engvall, E. and P. Perlmann. 1972. Enzyme-Linked Immunoasorbent Assay ELISA. III. Quantitation of Anti-Immunoglobulins in Antigen-Coated Tubes. J. Immunol. 109: 129-135. 10. Gut, J. P. 1985. J. Clinical Microbiology Volume 21:346-352. 11. Harmsen, T. 1992. J. Clinical Microbiology Volume 30:2139-2144.