Mycoplasma pneumonia, Human, IgG, BioAssay(TM) ELISA Kit

The United States Biological Mycoplasma pneumonia, Human, IgG, BioAssay(TM) ELISA Kit is intended for the semi-quantitative or qualitative determination of IgG antibodies in sample serum to Mycoplasma pneumoniae for the determination of immunological experience. The United States Biological Mycoplasma pneumonia, Human, IgG, BioAssay(TM) ELISA Kit may be used to evaluate paired serum for the presence of seroconversions and a significant increase in specific IgG.\n\nMycoplasma pneumoniae is among the smallest of free-living organisms. In samples, it is usually found in the throat and lungs and is susceptible to antimicrobial agents that inhibit protein synthesis. The spread of mycoplasmal infections depends on close and prolonged contact between people, as it is transmitted by means of droplet nuclei from the respiratory tract.\n\nMycoplasmal infection may be asymptomatic, or may produce upper espiratory tract disease, or atypical pneumonia. The pneumonia is difficult to differentiate from viral diseases by clinical means alone. Laboratory tests such as isolation, Complement Fixation serology and ELISA serology are helpful as aids in the differentiation of Mycoplasma pneumoniae infection. \n\nKit Components:\n1. Microtiter Plate (Mycoplasma, FH strain, grown in PPLO broth, washed, detergent treated, and sonicated): 1x96 wells\n2. Serum Diluent: 1X30ml \n3. Cutoff Calibrator: 1X400ul \n4. Positive Control, High: 1X400ul\n5. Positive Control, Low: 1X400ul\n6. Negative Control: 1X400ul \n7. IgG (HRP) Goat x human: 1X16ml \n8 Tetramethylbenzidine (TMB): 1X15ml \n9. Wash Buffer, 20X: 1X50ml \n10 Stop Solution: 1X15ml (1N H2SO4) \n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

M9750-16

Size

1Kit

Applications

ELISA

Reactivities

Hum

References

1. Philip, A. 1980. Mycoplasmal pneumonia: Disease Characteristics and Laboratory Diagnosis. Lab Med. (11) 4:241. 2. Klein, J.O. 1983. Mycoplasmal Infections In: Infectious Diseases of the Fetus and Newborn Infant. J.S. Remington and J.O. Klein (Eds.). W.B. Saunders Co. pp.428-449. 3. Jacobs, E. ,et al., J Clin Microbiology. (23) 3:517-522. 4. Fischer, G. S., et al., Diagn. Microbiol Infect Dis. 4:139-145. 5. Engvall, E., K. Jonsson, and P. Perlman. 1971. Enzyme-Linked Immunosorbent Assay, (ELISA) Quantitative Assay of Immunoglobulin G. Immunochemistry. 8:871-874. 6. Engvall, E. and P. Perlman. 1971. Enzyme-Linked Immunosorbent Assay, ELISA. Protides of the Biological Fluids, H. Peeters, Ed., Proceedings of the Nineteenth Colloquium, Brugge Oxford. Pergamon Press. p. 553-556. 7. Engvall, E., et al., Biochem. Biophys. Acta., 251: 427-434 8. Van Weeman, B. K. and A.H.W.M. Schuurs. 1971. Immunoassay Using Antigen-Enzyme Conjugates. FEBS Letter. 15: 232-235. 9. Bakerman, S. 1980. Enzyme Immunoassays. Lab. Mgmt. August, p. 21-29. 10. Voller, A., and D. E. Bidwell. 1975. Brit. J. Exp. Pathology. 56:338-339. 11. Engvall, E. and P. Perlman. 1972. Enzyme-Linked Immunosorbent Assay, ELISA. III. Quantitation of Anti-Immunoglobulins in Antigen-Coated Tubes. J. Immunol. 109: 129-135. 12. Kenny, G. E., et al., J. Clin. Microbiology. (28) 9:2087-2093. 13. National Committee for Clinical Laboratory Standards. Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture Approved Standard. NCCLS. 14. CDC/NIH Manual. 1993. Biosafety in Microbiological and Biomedical Laboratories. 3rd Edition. pp. 9-12. U.S. Dept. of Health and Human Services, Public Health Service. 15. Medical Microbiology, MMWR Cse Definitions for Infectious Conditions Under Public Health Surveillance. May 2, 1997. Vol. 46. No. RR-10. 16. Clinical Microbiology Reviews. July 2002. Serologic Diagnosis. pp. 506-526. 15: 3.