Myosin, Cardiac, Heavy Chain (Alpha MHC, ASD3, CMD1S, CMH1, MGC138376, MGC138378, MPD1, MYH6, MYH7, MYHC, MyHC-alpha, MYHCA, MyHC-beta, MYHCB, Myosin Heavy Chain Cardiac Muscle alpha Isoform, Myosin Heavy Chain Cardiac Muscle beta Isoform, Myosin Heavy Po

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Cardiac MHC exists as two isoforms in humans, alpha-cardiac MHC and beta-cardiac MHC. These two isoforms are expressed in different amounts in the human heart. During normal physiology, beta-cardiac MHC is the predominant form, with the alpha-isoform contributing around only 7% of the total MHC. Mutations of the MHC genes are associated with several different dilated and hypertrophic cardiomyopathies.\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:100-1:2000\nImmunohistochemistry: 1:10-1:2000\nImmunohistochemistry (Frozen): 10ug/ml tissue treatment may reduce or destroy the myosin binding sites\nImmunohistochemistry(Paraffin): 1:400\nWestern Blot: 1:100-1:2000. Detects a band at ~223kD\nImmunocytochemistry (IF): 1:10-1:2000\nIndirect Immunofluorescence (Frozen): Binds to A-band of thick filament of cardiac and skeletal muscle myosin and indirect immunoperoxidase staining of Carnoy fixed tissues.\nOptimal dilutions to be determined by the researcher.\n\nAffinity Constants:\nHuman ventricular myosin heavy chains: 3.33 x10e8/M Human atrial myosin heavy chains: 1.48 x10e8/M \nHuman skeletal muscle myosin: 1.06 x10e8/M \nCanine cardiac myosin heavy chains: 2.06 x10e8/M\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

M9850-11C

Size

100ug

Applications

ELISA, ICC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Prc, Rabbit

Form

Supplied as a liquid in PBS, pH 7.4. No preservatives added.

P Type

Mab

Purity

Purified

Isotype

IgG1,k

Additional Info

Recognizes human myosin alpha and beta heavy chains. Reacts with heavy chains of human myosin isolated from: slow skeletal muscle, fast skeletal muscle, eye muscle, and streptococcal M5 peptide. (Myosin heavy chain is specific for cardiomyocytes.). It does not react with human platelet, smooth muscle myosin heavy chains, myosin light chains, myosin fragments circulating in blood of patients with myocardial infarction. Species Crossreactivity: bovine, mouse, rabbit, rat, canine and porcine.