A nitrilotriacetic acid derivative used as a metal chelating adsorbent for metal ion affinity chromatography. This method can be used for identification and rapid one-step purification of gene products expressed as fusion proteins with an oligo-hist
Melting Point:
113-119C
Solubility:
Methanol (sparingly), Water
Method for Determining Identity:
Proton NMR Spectroscopic Analysis
TLC Conditions:
SiO2; n-Butanol: Pyridine: Acetic Acid: Water; 4: 3: 1: 3; Visualized with Ninhydrin or KMnO4; Single spot; Rf=0.35
Storage and Stability:
May be stored at RT for short-term only. Long-term storage is recommended at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap
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