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NcoI

Cat no: N0554

NcoI

5'-C^C A T G G-3' \n3'-G G T A C^C-5'\n\nSource: \nNocardia corallina\n\nConcentration:\n10u/ul\n\nUnit Definition:\nOne unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37 degrees C in 50ul of assay buffer.\n\nSupplied With:\nR1625: Restriction Enzyme Buffer A, 10X Supplied as a liquid. 1x contains 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.\n\nStorage Buffer:\nSupplied as a liquid in 10mM Tris-HCl, pH 7.5, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol.\n\nDilution Buffer:\n10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N0554

Size

1000U

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