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NFkB2, p52, p100 (Nuclear Factor kappa B)

Cat no: N2301-39D

NFkB2, p52, p100 (Nuclear Factor kappa B)

NFkB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was\nsubsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB. NFkB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NkFB subunit\np65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by an inhibitor called IkB-a. IkB-a binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation, and Gel Supershift assay. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Secondary antibody used was peroxidase-conjugated anti-rabbit IgG. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2301-39D

Size

100ul

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.

P Type

Pab

Purity

Serum (delipidated and defibrinated)

Isotype

IgG

References

Wang, Y., et al. (2002) NFKB2 p100 is a pro-apoptotic protein with anti-oncogenic function. Nature Cell Biol. 4:11;888-893.\n

Additional Info

Recognizes human NFKB2 p52 and its precursor protein p100.

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