NHE2 (Na, H Exchanger 2)

Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. Five NHE isoforms (NHE1-5) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The COOH-terminals of NHE1, NHE2 and NHE3 are intracellular.\n\nNHE2 (rat 813 aa; human chromosome 2 and rat chromosome 9) has been implicated in volume regulation in renal inner medullary collecting duct cells. Its mRNA is found in kidney medulla, cortex, colon, jejunum, ileum, human jejunum, ileum, duodenum, stomach and adrenal glands (1).\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:100,000 using 5-10ng control peptide/well. Control peptide is N2016-06.\nWestern Blot: 1-10ug/ml using Chemiluminescence.\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2016-02

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Sardet C et al (1989) Cell 56, 271; Sardet C et al (1990) Science 247, 723; Tse CM et al (1991) Mol Cell Biochem. 125, 137; Orlowski J et a (1992) J Biol. Chem. 267, 9331; Chris Yun CH et al (1995) Am J Physiol. 269, G1-G11 (Review); Josette N and Pouyssegur J (1995) Am J Physiol. 268, C283-C296 \n(review); Yoshioka et al (1997) J Biol. Chem. 122, 641; Yamashita T et al (1992) J Biochem. 111, 162; Siczkowski M et al (1995) Cir. Res. 76, 825.

Additional Info

Recognizes rat NHE2. No significant homology exists with other NHE isoforms (NHE3-5) \nSpecies sequence homology: Human, mouse, bovine, porcine, rabbit: 95%; Hamster NHE1: 90%.