Nibrin, p95, (Nijmegen Breakage Syndrome, NBS1)

The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex. This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions.\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blotting, Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:200-1:500\nWestern Blotting: 1:5000-1:10,000. ~95kD\nImmunoprecipitation: Methanol-fixed IMR90 primary human fibroblasts, ~3-x10e6 cells. \n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPositive control: Hela whole cell extract, TK6 cell lysates

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SPECIFICATIONS

Catalog Number

N2498-03

Size

50ul

Applications

IF, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as serum , 0.02% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Carney, J.P., et al. The hMre11/hRad50 Protein Complex and Nijmegen Breakage Syndrome: Linkage of Double-Strand Break Repair to the Cellular DNA Damage Response. Cell. 93: 477-486, 1998. 2. Wang, et al. BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes & Development. 14: 927-939, 2000. 3. Lim, et al. ATM phosphorylates p95/nbs1 in an S-phase checkpoint pathway. Nature. 404: 613-617, 2000.\nGeneral References:\n1. Wang, et al. BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes & Development. 14: 927-939, 2000. 2. Franchitto, A., and P. Pichierri (2002) Bloom's syndrome protein is required for correct relocalization of RAD50/MRE11/NBS1 complex after replication fork arrest. The Journal of Cell Biology. DOI:10.1083/jcb.200110009. 3. Lee, W., et al. Independence of R/M/N Focus Formation and the Presence of Intact BRCA1. Science. 289: 11a, 2000. 4. Gatei, M., et al. ATM-dependent phosphorylation of nibrin in response to radiation exposure. Nature Genetics. 25: 115-119, 2000. 5. Stracker, T.H., etal. Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex. Nature. 418: 348-352, 2002. 6. Huang, Juren, et al. Reconstitution of the mammalian DNA double-strand break end-joining reaction reveals a reqirement for an Mre11/Rad50/NBS1-containing fraction. Oxford University Press. 30(3):667-674, 2002. 7. Buscemi, Giacomo., et al. Chk2 Activation Dependence on Nbs1 after DNA Damage. Molecular and Cellular Biology. 21(15):5214-5222, 2001.

Additional Info

Recognizes human p95 in Western Blot, immunofluorescence and immunoprecipitation. Does not work by western blotting in mouse.