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NKG2 Isoforms A, C, E (FITC)

Cat no: N2851-75S

NKG2 Isoforms A, C, E (FITC)

In mice, NKG2 subunits associate with CD94 to form heterodimers at the surface of natural killer (NK) cells. The CD94/NKG2 heterodimer is the receptor for a non-classical MHC class I ligand, which is Qa-1 in the mouse.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

N2851-75S

Size

100ug

Applications

FC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Vance, R. E. et al. (1999) Recognition of the Class Ib Molecule Qa-1b by Putative Activating Receptors CD94/NKG2C and CD94/NKG2E on Mouse Natural Killer Cells. J. Exp. Med. 190:1801-1812.2. Vance, R. E. et al. (2002) Implications of CD94 deficiency and monoallelic NKG2A expression for natural killer cell development and repertoire function. Proc. Natl. Acad. Sci. USA. 99:868-873.

Additional Info

Recognizes mouse NKG2A, NKG2C and NKG2E, which are isoforms of the NKG2 lectin-like family. Reported to block ligand binding to the receptor.

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