NKR2B4 (Natural Killer Cell Receptor 2B4, CD244, SLAMF4) (Carboxyfluorescein)

NKR2B4 is a 66kD type I transmembrane glycoprotein in the SLAM subgroup of the CD2 protein family. SLAM family proteins have an extracellular domain (ECD) with 2 or 4 Ig-like domains and at least 2 cytoplasmic immunoreceptor tyrosine-based switch motifs (ITSMs). NKR2B4 interacts with CD48, while other SLAM family proteins interact homophilically. Mature human 2B4 consists of a 208aa ECD with two Ig-like domains, a 21aa transmembrane segment and a 120aa cytoplasmic domain with four ITSMs. Three additional splice variants of human NKR2B4 have deletions of the short region between the Ig-like domains, the second Ig-like domain or a portion of the cytoplasmic tail. Within the ECD, human NKR2B4 shares 46% and 40% aa sequence identity with mouse and rat NKR2B4, respectively. The ECD of human NKR2B4 shares 17%-24% aa sequence identity with comparable regions of human CD2 family members BLAME, CD2F-10, CD84, CD229, CRACC, NTB-A and SLAM. NKR2B4 is expressed on all NK cells, gamma-delta T cells, monocytes, some CD4+ and CD8+ T cells and some dendritic cells. CD48 mediates 2B4+ cell interactions with nearly all hematopoietic cell types, including cells of the same type. NKR2B4 signaling cooperates with other receptor systems to either promote or inhibit NK and CD8+ T cell activation. The inhibitory activities are distinct from those of MHC I restricted inhibitory NK cell receptors. Ligation of NKR2B4 with antibodies or CD48 constructs can either directly trigger inhibitory signaling or disrupt an inhibitory interaction, leading to cellular activation. The inhibitory effect is associated with the long form of NKR2B4, while the activation is associated with the short form. NKR2B4 can also induce signaling through CD48. \n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\nRecommended Dilutions:\nFlow Cytometry: 10ul labels 10e5 cells or 50ul of packed whole blood\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Carboxyfluorescein conjugates are sensitive to light.

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