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NMD3, CT (60S Ribosomal Export Protein NMD3, hNMD3, CGI-07) (Azide free) (HRP)

Cat no: N2905-45-HRP

NMD3, CT (60S Ribosomal Export Protein NMD3, hNMD3, CGI-07) (Azide free) (HRP)

It has been suggested that NMD3 is a cytoplasmic factor required for a late cytoplasmic assembly step of the 60S subunit but is not a ribosomal protein. A mutation in NMD3 was found to be lethal in the absence of XRN1, which encodes the major cytoplasmic exoribonuclease responsible for mRNA turnover. The NMD3 protein sequence does not contain readily recognizable motifs of known function. However, NMD3 orthologues display an amino-terminal domain containing four repeats of Cx2C, reminiscent of zinc-binding proteins, implicated in nucleic acid binding or protein oligomerization.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze horseradish peroxidase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2905-45-HRP

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). Conjugated to HRP.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Trotta, C.R., et al., EMBO J. 22(11):2841-2851 (2003). 2. Ho, J.H. et al., Mol. Cell. Bio., 19(3):2389-2399 (1999).

Additional Info

Recognizes human NMD3. Species Crossreactivity: mouse.

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