NMDA 1 Receptor (NMDA R1, N-methyl-D-aspartate, N-methyl-D-aspartate Receptor Channel, subunit zeta-1 NR1, Glutamate [NMDA] Receptor subunit zeta 1, Ionotropic, N-methyl D-aspartate 1)

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4- isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.\n\nApplications: \nSuitable for use in Western blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 detects NMDAR1 on 10ug of rat brain lysate. Labels a single band at approximately 120kD corresponding to NMDAR1 in rat brain lysate.\nImmunohistochemistry: 1:1000-1:2000 with light microscopy. Tested with 4% paraformaldehyde or paraformaldehyde/glutaraldehyde fixed rat tissue. Optimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\n\nPositive Control: Brain tissue\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2904-31

Size

50ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid containing 0.05% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Liu, Xiao-Bo, et al. J. Neurosci., 25: 9124-9134 (2005). 2. Maatkamp, Arjen, et al., Eur. J. Neurosci., 20: 14-28 (2004). 3. Dalmau, J. et al., Lancet Neurology 7:1091-1098 (2008). 4. Jin-bian Liu, J.-B. et al., Neuroscience Letters 452:268-272 (2009).

Additional Info

Recognizes rat splice variants NR1-1a, NR1-1b, NR1-2a, NR1-2b at ~120kD. These appear to be the major splice variants expressed in rat brain. Reactivity in mouse is weak. No crossreaction with other glutamate receptor subunits. Species sequence homology: monkey, gerbil, rabbit, feline and fish.