NOTCH1 (Notch 1, Neurogenic Locus Notch Homolog Protein 1, hN1, Translocation-associated Notch Protein TAN-1, TAN1)

Notch1, also known as neurogenic locus notch homolog protein 1, is a large >270kD protein that functions as a receptor for the membrane ligands Jagged1, Jagged2 and Delta1 to regulate cell fate decisions. Upon ligand activation, the transmembrane Notch1 receptor is cleaved by TNF-alpha converting enzyme (TACE) to produce a membrane-associated intermediate fragment (NEXT). This fragment is further cleaved by presenilin-dependent gamma secretase to release a notch-derived peptide containing the intracellular fragment from the membrane. The released Notch intracellular fragment (NICD) translocates to the nucleus and forms a transcriptional complex with the RBP-J k transcriptional activator complex to alter differentiation, proliferation, and apoptotic programs. Notch 1 is highly expressed in the brain, lung, and thymus (CD4-CD8- cells and CD4-CD8+ cells) with lower levels of expression observed in the spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney and heart. The transmembrane Notch protein is a heterodimeric protein consisting of a C-terminal fragment and N-terminal fragment (probably linked by disulphide bonds) containing 5 ankyrin repeats, 36 EGF repeats, and 3 Notch/Lin repeats. Notch1 can be modified by phosphorylation. This monoclonal antibody reacts with the intracellular domain of mouse and human Notch1 and has been reported to have highest affinity for activated intracellular Notch1 and lower affinity for full-length unprocessed/heterodimeric Notch1 forms. This antibody does not recognize rat Notch1 or cross-react with Notch2, 3, or 4.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N5375-05E

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1,k

References

1. Huppert, S.S., et al. 2000. Nature 405:966. 2. Ray, W. J., et al. 1999. Proc. Natl. Acad. Sci. USA 96:3263. 3. DeStrooper, B., et al. 1999. Nature 398:518. 4. Sun, H., et al. 2007. J. Cell Biol. 177:647. (IHC) 5. Jundt, F., et al. 2008. Leukemia. 22:1587. (IHC) 6. Kopan, R., et al.i> 1993. J. Cell. Biol. 121:631. 7. Saxena, M. T., et al. 2001. J. Biol. Chem. 276:40268. 8. Mizutani, T., et al. 2001. Proc. Natl. Acad. Sci. USA 98:9026.

Additional Info

Recognizes human Notch 1. Species Crossreactivity: mouse.