NUDT5, Recombinant Human, (ADP-sugar Pyrophosphatase, HSPC115, hYSAH1, Nucleoside Diphosphate-linked Moiety X Motif 5, Nudix Motif 5, YSA1H)

ADPпїЅSugar Pyrophosphatase (NUDT5)is a member of the nudi x super family of enzymes. Members of this family are pyrophosphohydrol gases that act upon substrates with the general structure of a nucleoside(Nu) diphosphate (di) linked to another moiety, X (NDPпїЅX) to yield NMP plus PпїЅX (1).Human NUDT5 is a homodimeric enzyme present in the cytosol of most cell types (2). Glu166 and three magnesium ions are important for stabilizing the transition state during the hydrolysis of ADPR (3) . NUDT5 has been suggested to play a role in regulating the intracellular levels of ADPR by NO activation through ADP ribosyl at ion at cysteine residues of the enzyme in macrophages (4). It also may play defensive role against the mutagenesis induced by oxidized deoxyribonucl eosides (5,6).\n\nSource: \nRecombinant corresponding to Glu2-Phe219 from human ADP-Sugar Pyrophosphatase/NUDT5, N-terminal, Met, 6-His tag, expressed in E. coli.\n\nMolecular Weight: \n~25kD\n\nBiological Activity:\nMeasured by its ability to hydrolyze ADP-ribose to AMP and ribose-5-phosphate. The specific activty is >1,000 pmoles/min/ug, as measured under the described conditions. \n \nEndotoxin Level:\n <1EU/ug (LAL method) \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N7400-23

Size

10ug

Form

Supplied as a liquid in Tris, sodium chloride, EDTA, DTT, Glycerol.

Purity

~95% (SDS-PAGE)

References

1. McLennan, A.G. (2006) Cell Mol. Life Sci. 63:123. 2. Zha, M. et al . (2006) J. Mol. Biol. 364:1021. \n3. Zha, M. et al . (2008) J. Mol. Biol. 379:568. 4. Yu, H.N. et al . (2007) Biochem. Biophys. Res. Comm. \n354:764. 5. Hori, M. et al . (2010) Free Radic. Biol. Med. 48:1197. 6. Kamiya, H. et al . (2009) DNA Repair (Amst) 8:1250.