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NUP62, Recombinant, Human (Nuclear Pore Glycoprotein p62, 62kD Nucleoporin, Nucleoporin Nup62)

Cat no: 149549

NUP62, Recombinant, Human (Nuclear Pore Glycoprotein p62, 62kD Nucleoporin, Nucleoporin Nup62)

Protein p62 preferentially binds multiubiquitin chains and forms a novel cytoplasmic structure "sequestosome" which serves as a storage place for ubiquitinated proteins.\n\nSource:\nRecombinant protein corresponding to human p62, expressed in E. coli.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

149549

Size

25ug

Applications

ELISA, WB

Form

Supplied as a liquid in 20mM Tris-HCl, 150mM sodium chloride, 1mM EDTA, 5mM glutathione, 0.5mM GSSH, 10% glycerol.

Purity

~90% (Nickel NTA Affinity Chromatography)

References

1. Eng M Tan (1999). A Novel Cytoplasmic Protein with RNA-binding Motifs is an Autoantigen in Human Hepatocellular Carcinoma. J Exp Med 189(7):1101-1110. 2. Moscat J (2005). The p62 scaffold regulates nerve growth factor -induced NF-kapppa B activation by influencing TRAF6 polyubiquitination. J Biol Chem; 280(42):35625-9.

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