Oct-3 (Organic Cation Transporter 3)

Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic cations from the blood by an active process called "renal secretion". Functional studies have identified two distinct categories of organic cation transporters \n(OCTs): a system driven by transmembrane potential difference that governs the influx of cations, whereas the H+-gradient-dependent transport system may mediate the efflux of organic cations. Several multispecific, potential-sensitive transporters (OCT1-3) and H+-dependent transporters (OCTN1-3) have been cloned and characterized from various tissues. The OCT superfamily of proteins shares a high degree of sequence homology and display up to 12 transmembrane domains with cytoplasmic N and C-terminus.\n\nOCT3 (rat/mouse 551 aa; human 556 aa) shares 30% homology with OCT1 and 51% with OCT2. It is most abundant in rat placenta, and moderate in the intestine, heart, and brain. OCT3 expression is very low in kidney and lung and is undetectable in the liver. OCT3 recognizes TEA and guanidine along with other cations including neurotoxin, MPP0, neurotransmitter dopamine, and steroids. OCT3 has been identified as an extraneuronal monoamine transporter (uptake 2).\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml using ECL..\nELISA: 0.5-1ug/ml using 1ug/ml control peptide per well.\nOptimal dilutions to be determined by the researcher.\n\nControl peptide: O5402-05 \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

O5402-06

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, 0.1% BSA, pH 7.2.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Kekuda R et al (1998) J. Biol. Chem. 273, 15971-15979; Verhaagh S et al (1999) Genomics 55, 209-218; Gruendemann D et al (1998) Nat. Neurosci. 1, 349-351; Wu, X et al (1998) J. Biol. Chem. 273, 32776-32786; Koespell H (1998) Ann Rev. Physiol. 60, 243-266 (review).

Additional Info

Recognizes rat Oct-3.